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Natural killer cells and phagocytes such as neutrophils, monocytes, macrophages, and dendritic cells mainly mediate the innate immune responses. Almost all biologically important molecules such as proteins, sugars, lipids, and nucleic acids can act as antigens and induce an immune response 68. Humoral immune responses to these molecules can be T-cell dependent or T-cell independent.
Most proteins are T-cell dependent antigens that are processed and presented by antigen-presenting cells through major histocompatibility complex molecules to T cells 68. It has been reported that natural antibodies are present in the body against these proteins, probably to clear the metabolites of collagen and damaged autologus albumin 72,73. However, a small percentage of the pop- ulation is allergic to certain proteins such as gelatin, where an immediate hyper- sensitivity reaction mediated by IgE and in some cases delayed hypersensitivity reactions have been observed 74. Legumin nanoparticles were found to be nonimmunogenic, whereas legumin was found to produce antilegumin antibodies 75.
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Particularly, in case of solid tumors, the smaller nanoparticles can easily extravasate through the leaky tumor vasculature, whereas they are excluded from intact blood vessels in normal tissues. It has been estimated that the pore size of tumor vasculature varies from 200 to 600 nm, and this has been exploited for passive order Avana Online Without Prescription of nanopar- ticles to tumors 80. Furthermore, once they reach the tumor interstitium, larger molecules are retained due to the poor lymphatic drainage. Figure 7 schematically shows the combination of enhanced permeability and retention results in the pas- sive accumulation of nanoparticles in tumor tissues 81.
A further level of targeting can be achieved by attaching targeting ligands to the nanoparticles. Furthermore, albumin is known to accumulate in tumor tissues due to the leaky tumor vasculature 19. It is also believed that the albumin nanoparticles are taken up inside the cells by a glycoprotein receptor via caveolae 53. Most importantly, the patients can tolerate a higher paclitaxel dose with Abraxane. The neutral amino acids in gliadin are believed to interact with the intestinal mucosa through hydrogen bonding, whereas the lipophilic amino acids in gliadin can interact with the mucus through hydrophobic interactions 39. Intravitreal injection of this albumin nanoparticles achieved transgene expression after 48 hours. To this end, protein polymers from animal and plant sources are promising 88 Podaralla et al.
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Conformation of gelatin chains in aqueous solutions; part 1: A light and small angle neutron scattering study.